Concentration-adjustable micromixer using droplet injection into a microchannel

A novel micromixing technique that exploit a thrust of droplets into the mixing interface is developed. The technique enhances the mixing by injecting immiscible droplets in a mixing channel and the methodology enables a control of the mixing level simply by changing the droplet injection frequency. We experimentally characterize the mixing performance with various droplet injection frequencies, channel geometries, and diffusion coefficients. Consequently, it is revealed that the mixing level increases with the injection frequency, the droplet-diameter-to-channel-width ratio, and the diffusion coefficient. Moreover, the mixing level is found to be a linear function of the droplet volume fraction in the mixing section. The results suggest that the developed technique can produce a large amount of sample solution whose concentration is arbitrary and precisely controllable with a simple and stable operation.Comment: 12 + 3 pages, 6 + 4 figure

Model Checking with Program Slicing Based on Variable Dependence Graphs

In embedded control systems, the potential risks of software defects have been increasing because of software complexity which leads to, for example, timing related problems. These defects are rarely found by tests or simulations. To detect such defects, we propose a modeling method which can generate software models for model checking with a program slicing technique based on a variable dependence graph. We have applied the proposed method to one case in automotive control software and demonstrated the effectiveness of the method. Furthermore, we developed a software tool to automate model generation and achieved a 35% decrease in total verification time on model checking.Comment: In Proceedings FTSCS 2012, arXiv:1212.657

Membrane-damaging action of Clostridium perfringens alpha-toxin on phospholipid liposomes

AbstractThe effect of Clostridium perfringens alpha-toxin on multilamellar liposomes prepared from various phospholipids and cholesterol was investigated. The toxin induced carboxyfluorescein leakage from liposomes composed of the choline-containing phospholipids such as egg-yolk phosphatidylcholine and bovine brain sphingomyelin in a dose-dependent manner, but did not induce leakage from those liposomes composed of bovine brain phosphatidylethanol amine, egg-yolk phosphatidylserine or phosphatidylglycerol. The toxin-induced carboxyfluorescein leakage from egg-yolk phosphatidylcholine liposomes was increased by addition of divalent cations. The toxin induced carboxyfluorescein release from liposomes composed of phosphatidylcholine containing unsaturated fatty acyl residues or shorter chain length saturated fatty acyl residues (12 or 14 carbon atoms), but did not induce such release from liposomes composed of phosphatidylcholine containing saturated fatty acyl residues of between 16 and 20 carbon atoms. Furthermore, the toxin-induced carboxyfluorescein release decreased with increasing chain length of the acyl residues of phosphatidylcholine used. The toxin bound to liposomes composed of phospholipids which are hydrolyzed by the toxin, but did not bind to those composed of phospholipids which are not attacked by the toxin. The toxin-induced carboxyfluorescein release from liposomes composed of dipalmitoleoyl-l-α-phosphatidyl-choline and cholesterol and the toxin binding to the liposomes decreased with decreasing cholesterol contents. These observations suggest that the specific binding site formed by the choline-containing phospholipids and cholesterol, and membrane fluidity in liposomes are essential for the membrane-damaging activity of alpha-toxin

EFFECT OF KINEMATIC VARIABLES ON ENTRY PHASE IN COMPETITIVE SWIMMINGSTART

INTRODUCTION: Starting techniques are one of the most important factors for race performance in competitive swimming. Start phase (0-15m) is classified Block phase, Flight phase, Entry phase, Glide phase and Stroke phase. There are many study about effect of Kinematics and Kinetics variables on Block phase and Flight phase entry in competitive swimming start. However, there have been very few studies about the techniques of an Entry phase. The relationship between start techniques and performances of entry phase has not been clarified. Therefore, the purpose of this study was to investigate the effect of different entry methods on the performance of the entry phase

KICKING THE BACK PLATE OF THE STARTING BLOCK IMPROVES START PHASE PERFORMANCE IN COMPETITIVE SWIMMING

Kinematic characteristics and performance were evaluated during the start phase using two start techniques (kick start and track start) in 11 male elite collegiate swimmers. Trials were recorded using three synchronized video cameras shooting at 60 fps. Twodimensional video analysis was performed in the sagittal plane. Paired t-test was used to compare variables between the two techniques. The block time (kick start 0.70 ±0.04 s, track start 0.74 ±0.04 s,

Risk perceptions in Japanese SMEs:the role of Internet marketing capabilities in firm performance

Internet plays a very important role for the success of small- and medium-sized enterprises (SMEs). Many Japanese SMEs are risk-averse in adopting technological innovation such as the Internet. Others, despite these risk-related business norms, recognize the importance of technology and develop and deploy Internet marketing technology to compete in the current competitive environment. Drawing upon these contradicting practices among Japanese SMEs, this study investigates how Japanese SMEs risk perception impacts on the deployment of Internet marketing capabilities, the relationships between perception of risk, international mind-set, business networks, Internet marketing capabilities, and firm performance. This study makes two contributions. Firstly, there has been limited research investigating how Internet-related capabilities impact on firms’ performance. Secondly, there are few Internet-related studies in Japan. Thus, our emphasis on key antecedents and outcomes of Internet marketing capabilities among Japanese SMEs contributes significantly to our understanding of this unique contextual setting. The findings also provide some insights to managers/owners of Japanese SMEs that lower risk perception fosters adoption of the Internet and combined with other resources creates Internet marketing capabilities which results in higher performance